User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/07/05: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Bradford & cAMP precipitation</span>
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==Summary==
==Summary==
* Bradford (Enspire 2300 & Tecan Safire
* Bradford (Enspire 2300 & Tecan Safire)
* cAMP precipitation  
* cAMP precipitation  
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Revision as of 05:29, 29 July 2010

<html><style type="text/css"> .todo { color: red } .done { color: green} </style></html>

Bradford & cAMP precipitation <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Summary

  • Bradford (Enspire 2300 & Tecan Safire)
  • cAMP precipitation

Results

  • Sadly no difference could be seen between Enspire and the Tecan reader, and the RIPA buffer (not lysis), still yielded a very high signal
  • Everything was put equal to sample S1 and 1 mL of put to 30 μL of 8-AHA-cAMP agarose beads (3x)

Discussion

  • Maybe Pierce assay might be a better alternative to the Bradford assay is something in the buffer is interfering

Attachment

<html> <a href="http://openwetware.org/wiki/Image:Bradford_05072010.xls"> 

  <img src="http://openwetware.org/images/f/f8/Owwnotebook_icon.png" alt="" width="50" height="50">

</a> </html> Bradford results both Tecan and Enspire


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