User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/07/09: Difference between revisions

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==Notes==
==Notes==
-->
==Results==
==Results==
*{{todo| WHAT?}}
*Overnight autoradiograph of RII overlay did not change anything of the image of yesterday


==Discussion==
==Discussion==

Revision as of 09:17, 9 July 2010

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Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Summary

  • AKAP250 immunoprecipitation
  • Gradient (4-12%) SDS-PAGE (NuPage)
Gel loading hTERT D9 + HEK293 & HeLa -S see codes 21June2010
# 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
Gel
cAMP prec. 		X X Invitrogen Marker
(8 μL) 		HeLa -S
(20 μL) 		HEK293
(20 μL) 		Biorad Marker
(8 μL) 		C1
(20 μL) 		C2
(20 μL) 		C3
(20 μL) 		S1
(20 μL) 		S2
(20 μL) 		S3
(20 μL) 		X X X

Results

  • Overnight autoradiograph of RII overlay did not change anything of the image of yesterday

Discussion

  • DONE Ow..

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