User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/07/09: Difference between revisions
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* Ponceau shows bands at higher altitude, maybe look for AKAP-Lbc next to AKAP250? | * Ponceau shows bands at higher altitude, maybe look for AKAP-Lbc next to AKAP250? | ||
* Anoying that both markers are not running equal on every height, but HiMark at least has nice sharp band @ 160 kDa on NuPage gels. Although transfer was less perfect | * Anoying that both markers are not running equal on every height, but HiMark at least has nice sharp band @ 160 kDa on NuPage gels. Although transfer was less perfect | ||
* Coomassie staining shows not a 100% efficiency of transfer, and it shows bands in the control lanes that were not visible on Ponceau staining. Hopefully something did transfer | |||
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Revision as of 05:06, 10 July 2010
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