User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/07/09: Difference between revisions
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==Notes== | ==Notes== | ||
- | * HeLa cells had less sample put too gel, both HEK293 and HeLa are very slimy | ||
* Forgot to lyse the ordered HEK293 cells | |||
* Blot was blocked ON in 1% BSA in TBS-T | |||
* Gel was Coomassie stained ON | |||
==Results== | ==Results== | ||
*Overnight autoradiograph of RII overlay did not change anything of the image of yesterday | *Overnight autoradiograph of RII overlay did not change anything of the image of yesterday | ||
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==Discussion== | ==Discussion== | ||
* | * Ponceau shows bands at higher altitude, maybe look for AKAP-Lbc next to AKAP250? | ||
* Anoying that both markers are not running equal on every height, but HiMark at least has nice sharp band @ 160 kDa on NuPage gels. Although transfer was less perfect | |||
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==Related== | ==Related== | ||
===Related entries=== | ===Related entries=== |
Revision as of 10:28, 9 July 2010
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Summary
Notes
Results
Discussion
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