User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/07/10: Difference between revisions
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(Autocreate 2010/07/10 Entry for User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization) |
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==Summary== | ==Summary== | ||
* | * Destaining gel remains that was coomassie stained after blotting the cAMP precipitation samples | ||
* Putting the AKAP250 immunoprecipitation samples to gel and blot them | |||
* Gradient (4-12%) SDS-PAGE (NuPage) for cAMP Precipitations | |||
{| border="1" | |||
|+'''Gel loading hTERT D9 + HEK293 & HeLa -S see codes [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/21|21June2010]]''' | |||
! align="center" style="background:#f0f0f0;"|# | |||
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|- | |||
!Gel<br>cAMP prec. | |||
| align="center"|X | |||
| align="center"|X | |||
| align="center"|[[Media:HiMark_protein_marker_-_Invitrogen.jpg|Invitrogen Marker]]<br> (8 μL) | |||
| align="center"|HeLa -S <br>(20 μL) | |||
| align="center"|HEK293<br>(20 μL) | |||
| align="center"|[[Media:Biorad_-_Precision_Plus_Protein_Dual_Color_marker.jpg|Biorad Marker]]<br> (8 μL) | |||
| align="center"|C1<br>(20 μL) | |||
| align="center"|C2<br>(20 μL) | |||
| align="center"|C3<br>(20 μL) | |||
| align="center"|S1<br>(20 μL) | |||
| align="center"|S2<br>(20 μL) | |||
| align="center"|S3<br>(20 μL) | |||
| align="center"|X | |||
| align="center"|X | |||
| align="center"|X | |||
|- | |||
|} | |||
*After this gel has been blot it will be blocked and then together with the other blot treated with antiAKAP250 antibody | |||
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==Materials & Methods== | ==Materials & Methods== | ||
Revision as of 04:39, 10 July 2010
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