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Project name
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Summary
- Destaining gel remains that was coomassie stained after blotting the cAMP precipitation samples
- Putting the AKAP250 immunoprecipitation samples to gel and blot them
- Gradient (4-12%) SDS-PAGE (NuPage) for cAMP Precipitations
Gel loading hTERT D9 + HEK293 & HeLa -S see codes 21June2010
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Gel cAMP prec.
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X
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X
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Invitrogen Marker (8 μL)
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HeLa -S (20 μL)
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HEK293 (20 μL)
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Biorad Marker (8 μL)
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C1 (20 μL)
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C2 (20 μL)
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C3 (20 μL)
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S1 (20 μL)
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S2 (20 μL)
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S3 (20 μL)
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X
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X
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X
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- After this gel has been blot it will be blocked and then together with the other blot treated with antiAKAP250 antibody
Notes
- During wash steps of co-ip centrifuge steps were performed at 4400 rpm instead of 2500 rpm until the third wash step
- Supernatant of ON incubation for co-ip was saved, "NAIP 250 9.7.10" stored @ -20 °C
- Made new MES NuPage buffer
Results
- Coomassie staining was put together with the ponceau S staining of yesterday
- Ponceau staining of AKAP250 Co-IP blot
Discussion
- Very peculiar that the Ponceau staining gives the exact same image as the cAMP precipitations.
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