User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/07/12

From OpenWetWare

< User:Wilfred J. Poppinga | Notebook | cAMP compartmentalization | 2010 | 07
Revision as of 08:40, 29 July 2010 by Wilfred J. Poppinga (Talk | contribs)
(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)
Jump to: navigation, search

AKAP250 (scbt) immunoblot AKAP250 Co-IP Main project page
Previous entry      Next entry

Summary

  • Washing the AKAP250 CO-IP Blot and adding secondary antibody
  • Adding AKAP250 antibody ZDE10030 animal 122 antibody (Eurogentech antibody service) to cAMP precipitation blot blocked in blocking buffer + 1 μL/mL Azide (20%stock)

Results

  • Immunoblot for AKAP250 on AKAP250 co-ip western blot

Discussion

  • Same as yesterday for the cAMP precipitation, can not really see any Gravin signals. Maybe very faint in S2
  • Perhaps due to its aiming at the C-terminal the santa cruz C-16 antibody can not detect the protolytic fragment that might be seen on the ponceau of this membrane


Personal tools