User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/07/29: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">PKA-Co-IP gel</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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==Summary== | ==Summary== | ||
* | * 4-12% Gradient NuPage Gel | ||
{| border="1" | |||
|+'''Gel loading hTERT D9 + HEK293 & HeLa -S see codes [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/21|21June2010]]''' | |||
! align="center" style="background:#f0f0f0;"|# | |||
! align="center" style="background:#f0f0f0;"|<u>1</u> | |||
! align="center" style="background:#f0f0f0;"|<u>2</u> | |||
! align="center" style="background:#f0f0f0;"|<u>3</u> | |||
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! align="center" style="background:#f0f0f0;"|<u>6</u> | |||
! align="center" style="background:#f0f0f0;"|<u>7</u> | |||
! align="center" style="background:#f0f0f0;"|<u>8</u> | |||
! align="center" style="background:#f0f0f0;"|<u>9</u> | |||
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! align="center" style="background:#f0f0f0;"|<u>14</u> | |||
! align="center" style="background:#f0f0f0;"|<u>15</u> | |||
|- | |||
!Gel<br>PKA Co-IP | |||
| align="center"|X | |||
| align="center"|X | |||
| align="center"|[[Media:HiMark_protein_marker_-_Invitrogen.jpg|Invitrogen Marker]]<br> (8 μL) | |||
| align="center"|HeLa -S <br>(20 μL) | |||
| align="center"|HEK293<br>(20 μL) | |||
| align="center"|[[Media:Biorad_-_Precision_Plus_Protein_Dual_Color_marker.jpg|Biorad Marker]]<br> (8 μL) | |||
| align="center"|C1<br>(20 μL) | |||
| align="center"|C2<br>(20 μL) | |||
| align="center"|C3<br>(20 μL) | |||
| align="center"|S1<br>(20 μL) | |||
| align="center"|S2<br>(20 μL) | |||
| align="center"|S3<br>(20 μL) | |||
| align="center"|X | |||
| align="center"|X | |||
| align="center"|X | |||
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==Materials & Methods== | ==Materials & Methods== | ||
Revision as of 06:51, 23 August 2010
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 PKA-Co-IP gel
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Summary
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