User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/08/02: Difference between revisions
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** 35mm dishes for IF, 75.000 cells per well (3.862 mL cells + 12.138 mL S<sup>+</sup>) | ** 35mm dishes for IF, 75.000 cells per well (3.862 mL cells + 12.138 mL S<sup>+</sup>) | ||
*** IF: CTR/CSE, CTR+DMSO/CSE+DMSO & CTR+Ht31/CSE+Ht31 | *** IF: CTR/CSE, CTR+DMSO/CSE+DMSO & CTR+Ht31/CSE+Ht31 | ||
* | * Stop stimulation and Alamar Blue | ||
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==Materials & Methods== | ==Materials & Methods== | ||
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==Notes== | ==Notes== | ||
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==Results== | ==Results== | ||
* | *All cells treated with CSE are dead, CSE was too strong?? | ||
--!> | |||
==Discussion== | ==Discussion== | ||
*{{done}} Ow.. | *{{done}} Ow.. | ||
Revision as of 00:01, 3 August 2010
<html><style type="text/css"> .todo { color: red } .done { color: green} </style></html>
 Put cells to S0, Splitting cells, stopping stimulation
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Summary
Results
--!> Discussion
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