User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/08/04: Difference between revisions

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{|{{table}} width="800"
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Stop stimulation, put IF to S<sup>0</sup></span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Summary==
==Summary==
* {{todo}} Stop stimulation 11.55, 12.08
* {{todo}} Stop stimulation 11.55, 12.08
* {{todo}} Put IF cells to S<sup>0</sup>
* {{done}} Put IF & ELISA cells to S<sup>0</sup>
* {{todo}} Talk about repeating RII overlay
* {{done}} Talk about repeating RII overlay
** Remember to measure radioactivity when next RII is performed
* {{todo}} Talk about impact SSeCKS paper in respect to extra experiments
* {{todo}} Talk about impact SSeCKS paper in respect to extra experiments
** qPCR on Time range CSE stimulation? Enough to see effect without cell death?
** qPCR on Time range CSE stimulation? Enough to see effect without cell death?
** qPCR on concentration range CSE? Enough to see effect without cell death?
** qPCR on concentration range CSE? Enough to see effect without cell death?
* {{done}} Refresh medium new cells
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==Materials & Methods==
==Materials & Methods==

Revision as of 01:56, 4 August 2010

<html><style type="text/css"> .todo { color: red } .done { color: green} </style></html>

Stop stimulation, put IF to S0 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Summary

  • TODO Stop stimulation 11.55, 12.08
  • DONE Put IF & ELISA cells to S0
  • DONE Talk about repeating RII overlay
    • Remember to measure radioactivity when next RII is performed
  • TODO Talk about impact SSeCKS paper in respect to extra experiments
    • qPCR on Time range CSE stimulation? Enough to see effect without cell death?
    • qPCR on concentration range CSE? Enough to see effect without cell death?
  • DONE Refresh medium new cells