User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/08/04: Difference between revisions

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==Summary==
==Summary==
* {{todo}} Stop stimulation 11.55, 12.08
* {{todo}} Stop stimulation 11.55, 12.08
* {{todo}} Put IF cells to S<sup>0</sup>
* {{done}} Put IF & ELISA cells to S<sup>0</sup>
* {{done}} Talk about repeating RII overlay
* {{done}} Talk about repeating RII overlay
** Remember to measure radioactivity when next RII is performed
** Remember to measure radioactivity when next RII is performed
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** qPCR on Time range CSE stimulation? Enough to see effect without cell death?
** qPCR on Time range CSE stimulation? Enough to see effect without cell death?
** qPCR on concentration range CSE? Enough to see effect without cell death?
** qPCR on concentration range CSE? Enough to see effect without cell death?
* refresh medium
* {{done}} Refresh medium new cells
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==Materials & Methods==
==Materials & Methods==

Revision as of 01:56, 4 August 2010

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Summary

  • TODO Stop stimulation 11.55, 12.08
  • DONE Put IF & ELISA cells to S0
  • DONE Talk about repeating RII overlay
    • Remember to measure radioactivity when next RII is performed
  • TODO Talk about impact SSeCKS paper in respect to extra experiments
    • qPCR on Time range CSE stimulation? Enough to see effect without cell death?
    • qPCR on concentration range CSE? Enough to see effect without cell death?
  • DONE Refresh medium new cells