User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/08/09: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Isolate Protein/RNA Jurkat cells, Codes IF</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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==Summary== | ==Summary== | ||
* | * Isolate proteins and RNA from Jurkat cells (P7) | ||
** Centrifuge 5 min. 300xg to collect the cells | |||
** Instead of 0.2 mL/mL TRIzol; 400 μL for the 6 mL TRIzol | |||
** 2 mL of isopropanol (instead of 0.5 mL/mL TRIzol | |||
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==Materials & Methods== | ==Materials & Methods== | ||
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===Same actions=== | ===Same actions=== | ||
===Related topics=== | ===Related topics=== | ||
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==Attachment== | ==Attachment== | ||
<html> | <html> | ||
<a href="http://openwetware.org/wiki/Image: | <a href="http://openwetware.org/wiki/Image:IF_09082010_D9_P29.xls"> | ||
<img src="http://openwetware.org/images/f/f8/Owwnotebook_icon.png" alt="" width="50" height="50"> | <img src="http://openwetware.org/images/f/f8/Owwnotebook_icon.png" alt="" width="50" height="50"> | ||
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</html> [[Media: | </html> [[Media:IF_09082010_D9_P29.xls| IF D9 P29]] | ||
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<a href="http://openwetware.org/wiki/Image:09082010_jurkat_rnapdf.pdf"> | |||
<img src="http://openwetware.org/images/f/f8/Owwnotebook_icon.png" alt="" width="50" height="50"> | |||
</a> | |||
</html> [[Media:09082010_jurkat_rnapdf.pdf| RNA concentration Jurkat cells]] | |||
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Revision as of 02:09, 20 August 2010
<html><style type="text/css"> .todo { color: red } .done { color: green} </style></html>
 Isolate Protein/RNA Jurkat cells, Codes IF
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<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Summary
Attachment<html> <a href="http://openwetware.org/wiki/Image:IF_09082010_D9_P29.xls"> <img src="http://openwetware.org/images/f/f8/Owwnotebook_icon.png" alt="" width="50" height="50"> </a> </html> IF D9 P29 <html> <a href="http://openwetware.org/wiki/Image:09082010_jurkat_rnapdf.pdf"> <img src="http://openwetware.org/images/f/f8/Owwnotebook_icon.png" alt="" width="50" height="50"> </a> </html> RNA concentration Jurkat cells |
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