User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/08/09: Difference between revisions
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* Isolate proteins and RNA from Jurkat cells (P7) | * Isolate proteins and RNA from Jurkat cells (P7) | ||
** Centrifuge 5 min. 300xg to collect the cells | ** Centrifuge 5 min. 300xg to collect the cells | ||
** Instead of 0.2 mL/mL TRIzol; 400 μL for the 6 mL TRIzol | |||
** 2 mL of isopropanol (instead of 0.5 mL/mL TRIzol | |||
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==Materials & Methods== | ==Materials & Methods== | ||
Revision as of 09:50, 9 August 2010
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 Project name
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<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Summary
Attachment<html> <a href="http://openwetware.org/wiki/Image:IF_09082010_D9_P29.xls"> <img src="http://openwetware.org/images/f/f8/Owwnotebook_icon.png" alt="" width="50" height="50"> </a> </html> IF D9 P29 <html> <a href="http://openwetware.org/wiki/Image:09082010_jurkat_rnapdf.pdf"> <img src="http://openwetware.org/images/f/f8/Owwnotebook_icon.png" alt="" width="50" height="50"> </a> </html> RNA concentration Jurkat cells |
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