User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/08/10: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 14: Line 14:
*ELISA
*ELISA
** NOTE: Plate #1 well C1 received 200 µL of S<sup>0</sup> instead of CSE, well D1 received 400 µL and also did not receive CSE. Endvolumes were still 600 µL
** NOTE: Plate #1 well C1 received 200 µL of S<sup>0</sup> instead of CSE, well D1 received 400 µL and also did not receive CSE. Endvolumes were still 600 µL
{| border="1"
|+'''Gel loading hTERT D9 + HEK293, HeLa -S & Jurkat see codes [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/21|21June2010]]'''
! align="center" style="background:#f0f0f0;"|#
! align="center" style="background:#f0f0f0;"|<u>1</u>
! align="center" style="background:#f0f0f0;"|<u>2</u>
! align="center" style="background:#f0f0f0;"|<u>3</u>
! align="center" style="background:#f0f0f0;"|<u>4</u>
! align="center" style="background:#f0f0f0;"|<u>5</u>
! align="center" style="background:#f0f0f0;"|<u>6</u>
! align="center" style="background:#f0f0f0;"|<u>7</u>
! align="center" style="background:#f0f0f0;"|<u>8</u>
! align="center" style="background:#f0f0f0;"|<u>9</u>
! align="center" style="background:#f0f0f0;"|<u>10</u>
! align="center" style="background:#f0f0f0;"|<u>11</u>
! align="center" style="background:#f0f0f0;"|<u>12</u>
! align="center" style="background:#f0f0f0;"|<u>13</u>
! align="center" style="background:#f0f0f0;"|<u>14</u>
! align="center" style="background:#f0f0f0;"|<u>15</u>
|-
!Gel<br>cAMP prec.
| align="center"|X
| align="center"|X
| align="center"|[[Media:HiMark_protein_marker_-_Invitrogen.jpg|Invitrogen Marker]]<br> (8 μL)
| align="center"|HeLa -S <br>(20 μL)
| align="center"|HEK293<br>(20 μL)
| align="center"|[[Media:Biorad_-_Precision_Plus_Protein_Dual_Color_marker.jpg|Biorad Marker]]<br> (8 μL)
| align="center"|C1<br>(20 μL)
| align="center"|C2<br>(20 μL)
| align="center"|C3<br>(20 μL)   
| align="center"|S1<br>(20 μL)
| align="center"|S2<br>(20 μL)
| align="center"|S3<br>(20 μL)
| align="center"|X
| align="center"|X
| align="center"|X
|-
|}
<!--
<!--
==Materials & Methods==
==Materials & Methods==

Revision as of 03:42, 10 August 2010

<html><style type="text/css"> .todo { color: red } .done { color: green} </style></html>

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Summary

  • 4.450 mg st-Ht31 was diluted in 159 µL DMSO
  • ELISA
    • NOTE: Plate #1 well C1 received 200 µL of S0 instead of CSE, well D1 received 400 µL and also did not receive CSE. Endvolumes were still 600 µL
Gel loading hTERT D9 + HEK293, HeLa -S & Jurkat see codes 21June2010
# 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
Gel
cAMP prec. 		X X Invitrogen Marker
(8 μL) 		HeLa -S
(20 μL) 		HEK293
(20 μL) 		Biorad Marker
(8 μL) 		C1
(20 μL) 		C2
(20 μL) 		C3
(20 μL) 		S1
(20 μL) 		S2
(20 μL) 		S3
(20 μL) 		X X X


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/08/10&oldid=442990"