User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/08/10: Difference between revisions

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! align="center" style="background:#f0f0f0;"|<u>11</u>
! align="center" style="background:#f0f0f0;"|<u>11</u>
! align="center" style="background:#f0f0f0;"|<u>12</u>
! align="center" style="background:#f0f0f0;"|<u>12</u>
! align="center" style="background:#f0f0f0;"|<u>13</u>
! align="center" style="background:#f0f0f0;"|<u>14</u>
! align="center" style="background:#f0f0f0;"|<u>15</u>
|-
|-
!Gel<br>cAMP prec.
!Gel<br>cAMP prec.
| align="center"|X
| align="center"|X
| align="center"|[[Media:HiMark_protein_marker_-_Invitrogen.jpg|Invitrogen Marker]]<br> (8 μL)
| align="center"|[[Media:HiMark_protein_marker_-_Invitrogen.jpg|Invitrogen Marker]]<br> (8 μL)
| align="center"|Jurkat (LYSATE) <br>(20 μL)
| align="center"|HeLa -S <br>(20 μL)
| align="center"|HeLa -S <br>(20 μL)
| align="center"|HEK293<br>(20 μL)
| align="center"|HEK293<br>(20 μL)
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| align="center"|S2<br>(20 μL)
| align="center"|S2<br>(20 μL)
| align="center"|S3<br>(20 μL)
| align="center"|S3<br>(20 μL)
| align="center"|X
| align="center"|X
| align="center"|X
| align="center"|X
|-
|-

Revision as of 06:33, 10 August 2010

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Summary

  • 4.450 mg st-Ht31 was diluted in 159 µL DMSO
  • ELISA
    • NOTE: Plate #1 well C1 received 200 µL of S0 instead of CSE, well D1 received 400 µL and also did not receive CSE. Endvolumes were still 600 µL
Gel loading hTERT D9 + HEK293, HeLa -S & Jurkat see codes 21June2010
# 1 2 3 4 5 6 7 8 9 10 11 12
Gel
cAMP prec. 		Invitrogen Marker
(8 μL) 		Jurkat (LYSATE)
(20 μL) 		HeLa -S
(20 μL) 		HEK293
(20 μL) 		Biorad Marker
(8 μL) 		C1
(20 μL) 		C2
(20 μL) 		C3
(20 μL) 		S1
(20 μL) 		S2
(20 μL) 		S3
(20 μL) 		X


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