User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/08/10: Difference between revisions

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** NOTE: Plate #1 well C1 received 200 µL of S<sup>0</sup> instead of CSE, well D1 received 400 µL and also did not receive CSE. Endvolumes were still 600 µL
** NOTE: Plate #1 well C1 received 200 µL of S<sup>0</sup> instead of CSE, well D1 received 400 µL and also did not receive CSE. Endvolumes were still 600 µL
{| border="1"
{| border="1"
|+'''Gel loading hTERT D9 + HEK293, HeLa -S & Jurkat see codes [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/21|21June2010]]'''
|+'''4-12% Gradient NuPage Gel loading hTERT D9 + HEK293, HeLa -S & Jurkat see codes [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/21|21June2010]]'''
! align="center" style="background:#f0f0f0;"|#
! align="center" style="background:#f0f0f0;"|#
! align="center" style="background:#f0f0f0;"|<u>1</u>
! align="center" style="background:#f0f0f0;"|<u>1</u>

Revision as of 06:34, 10 August 2010

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Summary

  • 4.450 mg st-Ht31 was diluted in 159 µL DMSO
  • ELISA
    • NOTE: Plate #1 well C1 received 200 µL of S0 instead of CSE, well D1 received 400 µL and also did not receive CSE. Endvolumes were still 600 µL
4-12% Gradient NuPage Gel loading hTERT D9 + HEK293, HeLa -S & Jurkat see codes 21June2010
# 1 2 3 4 5 6 7 8 9 10 11 12
Gel
cAMP prec.
Invitrogen Marker
(8 μL)
Jurkat (LYSATE)
(20 μL)
HeLa -S
(20 μL)
HEK293
(20 μL)
Biorad Marker
(8 μL)
C1
(20 μL)
C2
(20 μL)
C3
(20 μL)
S1
(20 μL)
S2
(20 μL)
S3
(20 μL)
X