User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/08/10: Difference between revisions

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==Notes==
==Notes==
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==Results==
==Results==
*{{todo| WHAT?}}
*Ponceau staining cAMP precipitation blot (4-12% NuPage)
 
[[Image:100810_ponceau_cAMP_%26_lysate2s.TIF|500px]]
==Discussion==
==Discussion==
*{{done}} Ow..
*{{done}} Ow..
 
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==Related==
==Related==
===Related entries===
===Related entries===

Revision as of 01:36, 11 August 2010

<html><style type="text/css"> .todo { color: red } .done { color: green} </style></html>

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Summary

  • 4.450 mg st-Ht31 was diluted in 159 µL DMSO
  • ELISA
    • NOTE: Plate #1 well C1 received 200 µL of S0 instead of CSE, well D1 received 400 µL and also did not receive CSE. Endvolumes were still 600 µL
4-12% Gradient NuPage Gel loading hTERT D9 + HEK293, HeLa -S & Jurkat see codes 21June2010
# 1 2 3 4 5 6 7 8 9 10 11 12
Gel
cAMP prec. 		Invitrogen Marker
(8 μL) 		Jurkat (LYSATE)
(20 μL) 		HeLa -S
(20 μL) 		HEK293
(20 μL) 		Biorad Marker
(8 μL) 		C1
(20 μL) 		C2
(20 μL) 		C3
(20 μL) 		S1
(20 μL) 		S2
(20 μL) 		S3
(20 μL) 		X

Results

  • Ponceau staining cAMP precipitation blot (4-12% NuPage)

Discussion

  • DONE Ow..


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