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Project name
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Summary
- 4.450 mg st-Ht31 was diluted in 159 µL DMSO
- ELISA
- NOTE: Plate #1 well C1 received 200 µL of S0 instead of CSE, well D1 received 400 µL and also did not receive CSE. Endvolumes were still 600 µL
1.100810
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'
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S0+DMSO
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S0+DMSO
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S0+DMSO
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st-Ht31 (50 µM)
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st-Ht31 (50 µM)
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st-Ht31 (50 µM)
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1 |
2 |
3 |
4 |
5 |
6
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CTR |
A |
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CSE (15%) |
B |
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CSE+db-cAMP (500 µM) |
C |
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CSE+ mPKI (400 nM) |
D |
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02.100810
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Control
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8Pcpt
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6Bnz
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Ht31+6Bnz
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DMSO
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Ht31
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1 |
2 |
3 |
4 |
5 |
6
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CTR |
A |
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CTR |
B |
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CSE |
C |
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CSE |
D |
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4-12% Gradient NuPage Gel loading hTERT D9 + HEK293, HeLa -S & Jurkat see codes 21June2010
#
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1
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2
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3
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4
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5
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6
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7
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8
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9
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10
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11
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12
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Gel cAMP prec.
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Invitrogen Marker (8 μL)
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Jurkat (LYSATE) (20 μL)
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HeLa -S (20 μL)
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HEK293 (20 μL)
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Biorad Marker (8 μL)
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C1 (20 μL)
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C2 (20 μL)
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C3 (20 μL)
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S1 (20 μL)
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S2 (20 μL)
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S3 (20 μL)
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X
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Results
- Ponceau staining cAMP precipitation blot (4-12% NuPage)
- Coomassie staining of the gel AFTER blot
Discussion
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