User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/08/11

From OpenWetWare

< User:Wilfred J. Poppinga | Notebook | cAMP compartmentalization | 2010 | 08
Revision as of 05:12, 20 August 2010 by Wilfred J. Poppinga (Talk | contribs)
(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)
Jump to: navigation, search

Jurkat qPCR results, Primary antibody WB, Ending stimulation ELISA Main project page
Previous entry      Next entry

Summary

  • Western blot of yesterday was cut in 3 and incubated with primary antibody
    • AKAP450 (mouse, BD Bioscience, 611518)
    • RIIα(mouse, BD Bioscience, 612243)
    • AKAP95 (mouse, Transduction Laboratories, 610995)
  • GAPDH qPCR
    • As internal control also GAPDH need to be measured,
    • 20 µL reaction volume, as given by the machine
    • Human GAPDH primer (Applied biosystems)
    • Taqman Gene expression master mix

Attachment

qPCR Jurkat expression AKAP12, AKAP9, GAPDH

ELISA VIABILITY STAINING ONLY (Alamar blue)


Personal tools