User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/08/12

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Summary

  • 2nd Antibody (mouse) on WB, 1:1000 dilution (SHOULD BE 1:10.000)
  • RIPA (Lysis) Buffer (per 1 mL)
    • 1.06 mg β-Glycerolphosphate
    • 1 mL RIPA buffer
    • 8 μL Protease inhibitor mix
    • 1 μL NaF (1 M)
    • 10 μL NaVO3 (10 mM)
    • 12.5 μL PMSF
  • Lysate HEK293 Cells
  • CONVOCAL MICROSCOPY! together with Beate
  • TODO Isolate RNA of HEK293 Cells

Results

  • Western blot

Discussion

  • DONE Ow..


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