User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/08/16: Difference between revisions
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==Summary== | ==Summary== | ||
* SDS-PAGE | * 2x SDS-PAGE | ||
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|+'''4-12% Gradient NuPage Gel loading LYSATES hTERT D9 + HEK293, HeLa -S & Jurkat see codes [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/21|21June2010]]''' | |+'''4-12% Gradient NuPage Gel loading LYSATES hTERT D9 + HEK293, HeLa -S & Jurkat see codes [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/21|21June2010]]''' | ||
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*NOTE: After 60 min. the SDS-PAGE was put to ice and the voltage was turned from 200 V to 100 V, to latter because of limitation in the amount of people running wet blots. | *NOTE: After 60 min. the SDS-PAGE was put to ice and the voltage was turned from 200 V to 100 V, to latter because of limitation in the amount of people running wet blots. | ||
* Both gels were blotted using the NuPage wetblot system, 2 h @ 30 V | |||
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==Materials & Methods== | ==Materials & Methods== | ||
Revision as of 07:20, 16 August 2010
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