User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/08/16: Difference between revisions
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*NOTE: After 60 min. the SDS-PAGE was put to ice and the voltage was turned from 200 V to 100 V, to latter because of limitation in the amount of people running wet blots. | **NOTE: After 60 min. the SDS-PAGE was put to ice and the voltage was turned from 200 V to 100 V, to latter because of limitation in the amount of people running wet blots. | ||
* Both gels were blotted using the NuPage wetblot system, 2 h @ 30 V | **Both gels were blotted using the NuPage wetblot system, 2 h @ 30 V | ||
* Cells were splitted to D9 P28 | |||
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==Materials & Methods== | ==Materials & Methods== |
Revision as of 07:21, 16 August 2010
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