User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/08/16: Difference between revisions

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*NOTE: After 60 min. the SDS-PAGE was put to ice and the voltage was turned from 200 V to 100 V, to latter because of limitation in the amount of people running wet blots.
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==Materials & Methods==
==Materials & Methods==

Revision as of 02:50, 16 August 2010

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Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Summary

  • SDS-PAGE
4-12% Gradient NuPage Gel loading LYSATES hTERT D9 + HEK293, HeLa -S & Jurkat see codes 21June2010
# 1 2 3 4 5 6 7 8 9 10
Gel
cAMP prec.
Invitrogen Marker
(8 μL)
Jurkat
(20 μL)
HEK293
(20 μL)
Biorad Marker
(8 μL)
S1
(20 μL)
S2
(20 μL)
S3
(20 μL)
C1
(20 μL)
C2
(20 μL)
C3
(20 μL)
  • NOTE: After 60 min. the SDS-PAGE was put to ice and the voltage was turned from 200 V to 100 V, to latter because of limitation in the amount of people running wet blots.