User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/08/20: Difference between revisions
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==Summary== | ==Summary== | ||
* | * Stripping solution | ||
**50 mL dH<sub>2</sub>O | |||
**5 mL Tris-Cl (1.0 M, pH 6.8) (Stacking gel buffer SDS-PAGE) | |||
** 1 g SDS | |||
** 150 mg DTT (Add later) | |||
#Prewarm buffer (w/o DTT) for 30 min. to 70 °C in waterbath | |||
#Add DTT just before adding membrane | |||
#Completely immerse membrane | |||
#Incubate 30 min @ 70 °C | |||
#Wash 3x with dH<sub>2</sub>O or TBS-T | |||
#Membrane can now be blocked again | |||
* qPCR AKAP8 | |||
** 25 μL | |||
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==Materials & Methods== | ==Materials & Methods== | ||
Revision as of 01:59, 20 August 2010
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