User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/08/21: Difference between revisions
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==Summary== | ==Summary== | ||
* SDS-PAGE Lysate samples | * SDS-PAGE Lysate samples | ||
** Forgot antioxidant, added after 1h 11 min.; power failure @ 2h 40 min. → continued to blot (2h, 30V) | |||
** Ponceau staining does not look very nice compared to others | |||
** Gel is coomassie stained | |||
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|+'''4-12% Gradient NuPage Gel loading LYSATES hTERT D9 + HEK293, HeLa -S & Jurkat see codes [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/21|21June2010]]''' | |+'''4-12% Gradient NuPage Gel loading LYSATES hTERT D9 + HEK293, HeLa -S & Jurkat see codes [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/21|21June2010]]''' | ||
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* Prepared cDNA | * Prepared cDNA | ||
* qPCR AKAP5 and GAPDH | * qPCR AKAP5 and GAPDH |
Revision as of 10:17, 21 August 2010
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Summary
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