User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/08/21: Difference between revisions
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* Prepared cDNA | * Prepared cDNA | ||
* qPCR AKAP5 and GAPDH | * qPCR AKAP5 and GAPDH | ||
** Not enough master mix for HEK293 and Jurkat controls | |||
[[Image:AKAP79_expression.jpg|500px]] | [[Image:AKAP79_expression.jpg|500px]] | ||
* Western blot 2<sup>nd</sup> Antibody (α-Rabbit) 1:10.000 | * Western blot 2<sup>nd</sup> Antibody (α-Rabbit) 1:10.000 | ||
** Blot #1 [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/08/16|16August2010]], Loading control was already performed [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/08/19|19August2010]] | |||
[[Image:21082010_Lysates_Immunoblot_AKAP250_and_79.tif|500px]] | |||
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==Materials & Methods== | ==Materials & Methods== | ||
Revision as of 10:28, 21 August 2010
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