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 Lysate SDS-PAGE, qPCR AKAP5, Western blot AKAP79 and Gravin
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Summary
- SDS-PAGE Lysate samples
- Forgot antioxidant, added after 1h 11 min.; power failure @ 2h 40 min. → continued to blot (2h, 30V)
- Ponceau staining does not look very nice compared to others
- Gel is coomassie stained
4-12% Gradient NuPage Gel loading LYSATES hTERT D9 + HEK293, HeLa -S & Jurkat see codes 21June2010
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10
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Gel
Lysates
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Invitrogen Marker
(8 μL)
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Jurkat
(20 μL)
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HEK293
(10 μL)
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Biorad Marker
(8 μL)
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C1
(20 μL)
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C2
(20 μL)
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C3
(20 μL)
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S1
(20 μL)
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S2
(20 μL)
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S3
(20 μL)
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- Prepared cDNA
- qPCR AKAP5 and GAPDH
- Not enough master mix for HEK293 and Jurkat controls
- Western blot 2nd Antibody (α-Rabbit) 1:10.000
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Lysate SDS-PAGE, qPCR AKAP5, Western blot AKAP79 and Gravin
