User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/08/23: Difference between revisions
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===Method=== | ===Method=== | ||
* Blocking blot of [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/08/21|21August2010]] for pre-IgG antibodies | * Blocking blot of [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/08/21|21August2010]] for pre-IgG antibodies | ||
** Blocking occured ~8 hours @ RT | |||
** Rabbit Pre-Immune serum taken just before immunisation was used(ZDE10030 animal 122 antibody (Eurogentech antibody service)) 1:1000 and incubated ON @ 4°C | |||
* SDS-PAGE of cAMP precipitation samples [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/21|21June2010]] | * SDS-PAGE of cAMP precipitation samples [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/21|21June2010]] | ||
** 10 min. 70 °C → RT | ** 10 min. 70 °C → RT |
Revision as of 09:53, 23 August 2010
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Summary
Materials & MethodsMaterialsMethod
Notes
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