User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/08/24: Difference between revisions
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==Summary== | ==Summary== | ||
* L314E, 10 μM | * L314E, 10 μM | ||
==Materials & Methods== | ==Materials & Methods== | ||
===Materials=== | ===Materials=== | ||
* | * 370 MBq/mL [γ<sup>32</sup>P]-ATP (18.5 MBq/500 µCi Hartmann Analytic) | ||
* Blots for RII Overlay | |||
** PKA Co-IP blot [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/29|29July2010]] used for RII overlay [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/08/03|03August2010]] | |||
** Lysate blot #2 [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/08/16|16August2010]] (for AKAP18δ-PP) | |||
** Lysate blot [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/08/13|13August2010]] (for AKAP18δ-L314E) | |||
** cAMP precipitation blot (2x) [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/08/23|23August2010]] (for AKAP18δ-L314E & AKAP18δ-PP) | |||
** Lysate blot (3 fragments) [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/08/18|18August2010]] used for immunoblot [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/08/19|19August2010]] and [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/08/21|21August2010]] | |||
** cAMP precipitation blot (3 fragments) [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/08/10|10August2010]] used for blot [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/08/12|12August2010]] & [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/08/14|14August2010]] | |||
===Method=== | ===Method=== | ||
* | * Western blot Rabbit pre-immune IgG | ||
** Secondary AB α-Rabbit (1:10.000) | |||
==Notes== | ==Notes== | ||
* | * PKA Co-IP blot was blocked in the isotope lab | ||
==Results== | ==Results== | ||
*{{todo| WHAT?}} | *{{todo| WHAT?}} | ||
==Discussion== | ==Discussion== | ||
*{{done}} Ow.. | *{{done}} Ow.. | ||
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==Related== | ==Related== | ||
===Related entries=== | ===Related entries=== |
Revision as of 02:40, 24 August 2010
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Summary
Materials & MethodsMaterials
Method
Notes
Results
Discussion
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