User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/08/24

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<html><style type="text/css"> .todo { color: red } .done { color: green} </style></html>

RII Overlay, Pre-IgG 2nd AB, CSE on HEK293, ASMC to S0, Split cells w. Mileh <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Summary

  • L314E, 10 μM

Materials & Methods

Materials

Method

Western blot Rabbit pre-immune IgG

  • Secondary AB α-Rabbit (1:10.000), 1h @ RT

RII Overlay

Notes

RII overlay

  • PKA Co-IP blot was blocked in the isotope lab
  • Not all membranes were activated again, while they were maintained under moist conditions
  • Column had fallen dry and cracked, this was not noticed until after addition of sample. Separation appeared still succesful

Results

  • Rabbit Pre-immune Western blot

Discussion

  • DONE Ow..


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