User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/08/25

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RII Overlay all Main project page
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Summary

  • All HEK293 Cells died, realized that it had not been 15% but 45% CSE!! Experiment failed, no repeat due to time issues.
  • Aided Caroline in her Cotransin experiment with the ASMC
  • RII Overlay results
  • Purified Rabbit IgG I5006 Sigma Aldrich

Results

  • RII Overlay



Discussion

  • PKA Co-IP Appears still unsuccessful, at least RII subunits would be expected to be visible
  • Even AKAP18δ-PP seems to inhibit some RII binding, seeing as the signal is somewhat fainter compared to the untreated blots


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