User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/10/11

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==Summary==
==Summary==
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* Blocking membrane of [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/10/08|08October2010]] --> Incubating membrane with RIIα (sc-909) and Gravin (animal 122)dilution 1:500 ON @ 4°C
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* Blocking membrane of [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/10/08|08October2010]] Incubating membrane with RIIα (sc-909) and Gravin (animal 122)dilution 1:500 ON @ 4°C
* Preparation of 2 10% Tris-glycine acrylamide gels
* Preparation of 2 10% Tris-glycine acrylamide gels
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Revision as of 07:54, 11 October 2010

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Summary

  • Blocking membrane of 08October2010 → Incubating membrane with RIIα (sc-909) and Gravin (animal 122)dilution 1:500 ON @ 4°C
  • Preparation of 2 10% Tris-glycine acrylamide gels
10% SDS-PAGE Gel loading hTERT D9 + Jurkat, HEK293 & HeLa -S see codes 21June2010 S0 & C0 are of 7.5.2010
# 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
Gel
Lysates
X Fermantas high range Marker
(5 μL)
HEK293
(30 μL)
HeLa -S
(30 μL)
Jurkat
(30 μL)
Fermantas high range Marker
(10 μL)
S0
(30 μL)
S1
(30 μL)
S2
(30 μL)
S3
(30 μL)
C0
(30 μL)
C1
(30 μL)
C2
(30 μL)
C3
(30 μL)
X
  • Run at 200 V


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