User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/10/11

(Difference between revisions)

Revision as of 08:56, 11 October 2010

Project name

Blocking membrane of 08October2010 → Incubating membrane with RIIα (sc-909) and Gravin (animal 122)dilution 1:500 ON @ 4°C
Preparation of 2 10% Tris-glycine acrylamide gels
Dilution of samples, corrected to lowest concentration

**10% SDS-PAGE Gel loading hTERT D9 + Jurkat, HEK293 & HeLa -S see codes 21June2010 S0 & C0 are of 7.5.2010**
#	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15
Gel Lysates	X	Fermantas high range Marker (5 μL)	HEK293 (30 μL)	HeLa -S (30 μL)	Jurkat (30 μL)	Fermantas high range Marker (10 μL)	S0 (30 μL)	S1 (30 μL)	S2 (30 μL)	S3 (30 μL)	C0 (30 μL)	C1 (30 μL)	C2 (30 μL)	C3 (30 μL)	X

@@ Line 13: / Line 13: @@
 * Blocking membrane of [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/10/08|08October2010]] → Incubating membrane with RIIα (sc-909) and Gravin (animal 122)dilution 1:500 ON @ 4°C
 * Preparation of 2 10% Tris-glycine acrylamide gels
+* Dilution of samples, corrected to lowest concentration
+{| {{table}}
+| align="center" style="background:#f0f0f0;"|'''Sample'''
+| align="center" style="background:#f0f0f0;"|''' μL Sample'''
+| align="center" style="background:#f0f0f0;"|'''μL dH2O'''
+|-
+| HEK293 (12/08/2010)||61,5||88,5
+|-
+| HeLa -S (p17 (20.6.2010)||26,0||124,0
+|-
+| S0 (7.5.2010)||236,9||0,0
+|-
+| S1||52,1||97,9
+|-
+| S2||150,0||0,0
+|-
+| S3||53,8||96,2
+|-
+| C0 (7.5.2010)||2470,4||0,0
+|-
+| C1||59,6||90,4
+|-
+| C2||36,1||113,9
+|-
+| C3||106,3||43,7
+|}
 {| border="1"
 |+'''10% SDS-PAGE Gel loading hTERT D9 + Jurkat, HEK293 & HeLa -S see codes [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/21|21June2010]] S0 & C0 are of 7.5.2010'''