User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/10/11

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* Blocking membrane of [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/10/08|08October2010]] → Incubating membrane with RIIα (sc-909) and Gravin (animal 122)dilution 1:500 ON @ 4°C
* Blocking membrane of [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/10/08|08October2010]] → Incubating membrane with RIIα (sc-909) and Gravin (animal 122)dilution 1:500 ON @ 4°C
* Preparation of 2 10% Tris-glycine acrylamide gels
* Preparation of 2 10% Tris-glycine acrylamide gels
 +
* Dilution of samples, corrected to lowest concentration
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Sample'''
 +
| align="center" style="background:#f0f0f0;"|''' μL Sample'''
 +
| align="center" style="background:#f0f0f0;"|'''μL dH2O'''
 +
|-
 +
| HEK293 (12/08/2010)||61,5||88,5
 +
|-
 +
| HeLa -S (p17 (20.6.2010)||26,0||124,0
 +
|-
 +
| S0 (7.5.2010)||236,9||0,0
 +
|-
 +
| S1||52,1||97,9
 +
|-
 +
| S2||150,0||0,0
 +
|-
 +
| S3||53,8||96,2
 +
|-
 +
| C0 (7.5.2010)||2470,4||0,0
 +
|-
 +
| C1||59,6||90,4
 +
|-
 +
| C2||36,1||113,9
 +
|-
 +
| C3||106,3||43,7
 +
|}
{| border="1"
{| border="1"
|+'''10% SDS-PAGE Gel loading hTERT D9 + Jurkat, HEK293 & HeLa -S see codes [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/21|21June2010]] S0 & C0 are of 7.5.2010'''
|+'''10% SDS-PAGE Gel loading hTERT D9 + Jurkat, HEK293 & HeLa -S see codes [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/21|21June2010]] S0 & C0 are of 7.5.2010'''

Revision as of 08:56, 11 October 2010

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Summary

  • Blocking membrane of 08October2010 → Incubating membrane with RIIα (sc-909) and Gravin (animal 122)dilution 1:500 ON @ 4°C
  • Preparation of 2 10% Tris-glycine acrylamide gels
  • Dilution of samples, corrected to lowest concentration
Sample μL Sample μL dH2O
HEK293 (12/08/2010)61,588,5
HeLa -S (p17 (20.6.2010)26,0124,0
S0 (7.5.2010)236,90,0
S152,197,9
S2150,00,0
S353,896,2
C0 (7.5.2010)2470,40,0
C159,690,4
C236,1113,9
C3106,343,7
10% SDS-PAGE Gel loading hTERT D9 + Jurkat, HEK293 & HeLa -S see codes 21June2010 S0 & C0 are of 7.5.2010
# 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
Gel
Lysates
X Fermantas high range Marker
(5 μL)
HEK293
(30 μL)
HeLa -S
(30 μL)
Jurkat
(30 μL)
Fermantas high range Marker
(10 μL)
S0
(30 μL)
S1
(30 μL)
S2
(30 μL)
S3
(30 μL)
C0
(30 μL)
C1
(30 μL)
C2
(30 μL)
C3
(30 μL)
X
  • Run at 200 V


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