User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/10/12: Difference between revisions
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* Membranes were washed 3x 15min. separately depending on secondary antibody | * Membranes were washed 3x 15min. separately depending on secondary antibody | ||
* α-goat secondary antibody was diluted 1:10.000, α-rabbit & α-mouse 1:2000 --> Incubation for 2 hours | * α-goat secondary antibody was diluted 1:10.000, α-rabbit & α-mouse 1:2000 --> Incubation for 2 hours | ||
* Preparation of 2x 10% tris-glycine gels | * Old school imaging {{todo|RESULTS}} | ||
* Preparation of 2x 10% tris-glycine gels --> Put to 4°C | |||
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==Materials & Methods== | ==Materials & Methods== | ||
Revision as of 07:55, 12 October 2010
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