User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/10/12
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==Summary== | ==Summary== | ||
| - | * | + | * Membranes were washed 3x 15min. separately depending on secondary antibody |
| + | * α-goat secondary antibody was diluted 1:10.000, α-rabbit & α-mouse 1:2000 --> Incubation for 2 hours | ||
| + | * Old school imaging | ||
| + | * Preparation of 2x 10% tris-glycine gels --> Put to 4°C | ||
| + | [[Image:12102010_6%25_%26_10%25_gels_hTERT.tif| 500px]] | ||
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==Materials & Methods== | ==Materials & Methods== | ||
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