User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/10/12

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==Summary==
==Summary==
* Membranes were washed 3x 15min. separately depending on secondary antibody
* Membranes were washed 3x 15min. separately depending on secondary antibody
 +
* α-goat secondary antibody was diluted 1:10.000, α-rabbit & α-mouse 1:2000 --> Incubation for 2 hours
 +
* Old school imaging
 +
* Preparation of 2x 10% tris-glycine gels --> Put to 4°C
 +
[[Image:12102010_6%25_%26_10%25_gels_hTERT.tif| 500px]]
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==Materials & Methods==
==Materials & Methods==

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Summary

  • Membranes were washed 3x 15min. separately depending on secondary antibody
  • α-goat secondary antibody was diluted 1:10.000, α-rabbit & α-mouse 1:2000 --> Incubation for 2 hours
  • Old school imaging
  • Preparation of 2x 10% tris-glycine gels --> Put to 4°C


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