User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/10/12
From OpenWetWare
(Difference between revisions)
m (→Summary) |
(→Summary) |
||
| Line 13: | Line 13: | ||
* Membranes were washed 3x 15min. separately depending on secondary antibody | * Membranes were washed 3x 15min. separately depending on secondary antibody | ||
* α-goat secondary antibody was diluted 1:10.000, α-rabbit & α-mouse 1:2000 --> Incubation for 2 hours | * α-goat secondary antibody was diluted 1:10.000, α-rabbit & α-mouse 1:2000 --> Incubation for 2 hours | ||
| - | * Old school imaging | + | * Old school imaging |
* Preparation of 2x 10% tris-glycine gels --> Put to 4°C | * Preparation of 2x 10% tris-glycine gels --> Put to 4°C | ||
| + | [[12102010_6%25_%26_10%25_gels_hTERT.tif| 500px]] | ||
<!-- | <!-- | ||
==Materials & Methods== | ==Materials & Methods== | ||
Revision as of 04:02, 25 October 2010
 Project name
|  Main project page Previous entry Next entry
|
Summary
| |
