User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/10/12 - Revision history

Wilfred J. Poppinga: /* Summary */

2010-10-25T08:02:28Z

Summary

←Older revision		Revision as of 08:02, 25 October 2010
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	* Old school imaging		* Old school imaging
	* Preparation of 2x 10% tris-glycine gels --> Put to 4°C		* Preparation of 2x 10% tris-glycine gels --> Put to 4°C
-	[[12102010_6%25_%26_10%25_gels_hTERT.tif\| 500px]]	+	[[Image:12102010_6%25_%26_10%25_gels_hTERT.tif\| 500px]]
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	==Materials & Methods==		==Materials & Methods==

Wilfred J. Poppinga: /* Summary */

2010-10-25T08:02:17Z

Summary

←Older revision		Revision as of 08:02, 25 October 2010
Line 13:		Line 13:
	* Membranes were washed 3x 15min. separately depending on secondary antibody		* Membranes were washed 3x 15min. separately depending on secondary antibody
	* α-goat secondary antibody was diluted 1:10.000, α-rabbit & α-mouse 1:2000 --> Incubation for 2 hours		* α-goat secondary antibody was diluted 1:10.000, α-rabbit & α-mouse 1:2000 --> Incubation for 2 hours
-	* Old school imaging ~~{{todo\|RESULTS}}~~	+	* Old school imaging
	* Preparation of 2x 10% tris-glycine gels --> Put to 4°C		* Preparation of 2x 10% tris-glycine gels --> Put to 4°C
		+	[[12102010_6%25_%26_10%25_gels_hTERT.tif\| 500px]]
	<!--		<!--
	==Materials & Methods==		==Materials & Methods==

Wilfred J. Poppinga: /* Summary */

2010-10-12T14:55:14Z

Summary

←Older revision		Revision as of 14:55, 12 October 2010
Line 13:		Line 13:
	* Membranes were washed 3x 15min. separately depending on secondary antibody		* Membranes were washed 3x 15min. separately depending on secondary antibody
	* α-goat secondary antibody was diluted 1:10.000, α-rabbit & α-mouse 1:2000 --> Incubation for 2 hours		* α-goat secondary antibody was diluted 1:10.000, α-rabbit & α-mouse 1:2000 --> Incubation for 2 hours
-	* Preparation of 2x 10% tris-glycine gels	+	* Old school imaging {{todo\|RESULTS}}
		+	* Preparation of 2x 10% tris-glycine gels --> Put to 4°C
	<!--		<!--
	==Materials & Methods==		==Materials & Methods==

Wilfred J. Poppinga: /* Summary */

2010-10-12T09:31:58Z

Summary

←Older revision		Revision as of 09:31, 12 October 2010
Line 13:		Line 13:
	* Membranes were washed 3x 15min. separately depending on secondary antibody		* Membranes were washed 3x 15min. separately depending on secondary antibody
	* α-goat secondary antibody was diluted 1:10.000, α-rabbit & α-mouse 1:2000 --> Incubation for 2 hours		* α-goat secondary antibody was diluted 1:10.000, α-rabbit & α-mouse 1:2000 --> Incubation for 2 hours
		+	* Preparation of 2x 10% tris-glycine gels
	<!--		<!--
	==Materials & Methods==		==Materials & Methods==

Wilfred J. Poppinga: /* Summary */

2010-10-12T08:58:56Z

Summary

←Older revision		Revision as of 08:58, 12 October 2010
Line 12:		Line 12:
	==Summary==		==Summary==
	* Membranes were washed 3x 15min. separately depending on secondary antibody		* Membranes were washed 3x 15min. separately depending on secondary antibody
		+	* α-goat secondary antibody was diluted 1:10.000, α-rabbit & α-mouse 1:2000 --> Incubation for 2 hours
	<!--		<!--
	==Materials & Methods==		==Materials & Methods==

Wilfred J. Poppinga: /* Summary */

2010-10-12T08:07:03Z

Summary

←Older revision		Revision as of 08:07, 12 October 2010
Line 11:		Line 11:
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
	==Summary==		==Summary==
-	* Membranes were washed separately depending on secondary antibody	+	* Membranes were washed 3x 15min. separately depending on secondary antibody
	<!--		<!--
	==Materials & Methods==		==Materials & Methods==

Wilfred J. Poppinga: /* Summary */

2010-10-12T08:02:00Z

Summary

←Older revision		Revision as of 08:02, 12 October 2010
Line 11:		Line 11:
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
	==Summary==		==Summary==
-	* ~~Short summary~~	+	* Membranes were washed separately depending on secondary antibody
	<!--		<!--
	==Materials & Methods==		==Materials & Methods==

Wilfred J. Poppinga: Autocreate 2010/10/12 Entry for User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization

2010-10-12T08:01:15Z

Autocreate 2010/10/12 Entry for User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization

New page

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{|{{table}} width="800"
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Summary==
* Short summary


|}

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