User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/10/13
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==Summary== | ==Summary== | ||
| - | * Put secondary antibody on AKAP79 and GAPDH (SARA) (Mouse 1:2000) | + | * Put secondary antibody on AKAP79 and GAPDH (SARA) (Mouse 1:2000)--> SAVE ANTIBODY |
| + | * qPCR Patient material | ||
| + | ** NOTE: F6, F7 & F8 received separate mastermix etc. (7.5μL Mastermix, 0,75μL primer/probe, 6,45 μL RNAse free water) whereas the rest had 14.70 μL of the mastermix etc. (375μL Mastermix, 37,5μL primer/probe, 322,5 μL RNAse free water (50 reactions)) | ||
| + | * Quantify loading and reload? | ||
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==Materials & Methods== | ==Materials & Methods== | ||
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