User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/10/13

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Current revision (08:48, 13 October 2010) (view source)
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* Put secondary antibody on AKAP79 and GAPDH (SARA) (Mouse 1:2000)--> SAVE ANTIBODY
* Put secondary antibody on AKAP79 and GAPDH (SARA) (Mouse 1:2000)--> SAVE ANTIBODY
* qPCR Patient material
* qPCR Patient material
 +
** NOTE: F6, F7 & F8 received separate mastermix etc. (7.5μL Mastermix, 0,75μL primer/probe, 6,45 μL RNAse free water) whereas the rest had 14.70 μL of the mastermix etc. (375μL Mastermix, 37,5μL primer/probe, 322,5 μL RNAse free water (50 reactions))
 +
* Quantify loading and reload?
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==Materials & Methods==
==Materials & Methods==

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Summary

  • Put secondary antibody on AKAP79 and GAPDH (SARA) (Mouse 1:2000)--> SAVE ANTIBODY
  • qPCR Patient material
    • NOTE: F6, F7 & F8 received separate mastermix etc. (7.5μL Mastermix, 0,75μL primer/probe, 6,45 μL RNAse free water) whereas the rest had 14.70 μL of the mastermix etc. (375μL Mastermix, 37,5μL primer/probe, 322,5 μL RNAse free water (50 reactions))
  • Quantify loading and reload?


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