User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/10/13: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Summary==
==Summary==
* Put secondary antibody on AKAP79 and GAPDH (SARA) (Mouse 1:2000)
* Put secondary antibody on AKAP79 and GAPDH (SARA) (Mouse 1:2000)--> SAVE ANTIBODY
* qPCR Patient material
** NOTE: F6, F7 & F8 received separate mastermix etc. (7.5μL Mastermix, 0,75μL primer/probe, 6,45 μL RNAse free water) whereas the rest had 14.70 μL of the mastermix etc. (375μL Mastermix, 37,5μL primer/probe, 322,5 μL RNAse free water (50 reactions))
* Quantify loading and reload?
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==Materials & Methods==
==Materials & Methods==

Latest revision as of 19:54, 26 September 2017

<html><style type="text/css"> .todo { color: red } .done { color: green} </style></html>

Project name Main project page
Previous entry      Next entry

Summary

  • Put secondary antibody on AKAP79 and GAPDH (SARA) (Mouse 1:2000)--> SAVE ANTIBODY
  • qPCR Patient material
    • NOTE: F6, F7 & F8 received separate mastermix etc. (7.5μL Mastermix, 0,75μL primer/probe, 6,45 μL RNAse free water) whereas the rest had 14.70 μL of the mastermix etc. (375μL Mastermix, 37,5μL primer/probe, 322,5 μL RNAse free water (50 reactions))
  • Quantify loading and reload?


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