User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/10/14

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(Autocreate 2010/10/14 Entry for User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization)
Current revision (08:28, 14 October 2010) (view source)
(Summary)
 
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==Summary==
==Summary==
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* Short summary
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* qPCR for AKAP5 gene in 2nd batch of patient material, now with 50 cycles (PCR yesterday failed to late amplification)
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* Instead of 50 now a mastermix for 55 samples was prepared (Mastermix 412.5μL, Primer/probe 41.25μL, 316.25μL Nuclease free water)
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==Materials & Methods==
==Materials & Methods==

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Summary

  • qPCR for AKAP5 gene in 2nd batch of patient material, now with 50 cycles (PCR yesterday failed to late amplification)
  • Instead of 50 now a mastermix for 55 samples was prepared (Mastermix 412.5μL, Primer/probe 41.25μL, 316.25μL Nuclease free water)


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