User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/10/18

From OpenWetWare

< User:Wilfred J. Poppinga | Notebook | cAMP compartmentalization | 2010 | 10(Difference between revisions)
Jump to: navigation, search
(Autocreate 2010/10/18 Entry for User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization)
Current revision (08:35, 19 October 2010) (view source)
m (Summary)
 
(One intermediate revision not shown.)
Line 11: Line 11:
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Summary==
==Summary==
-
* Short summary
+
* Loading 10% gel
 +
** Loading volume was corrected according to the quantification of previous blot to obtain equal loading
 +
{| border="1"
 +
|+'''10% SDS-PAGE Gel loading hTERT D9 + Jurkat, HEK293 & HeLa -S see codes [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/21|21June2010]] S0 & C0 are of 7.5.2010'''
 +
! align="center" style="background:#f0f0f0;"|#
 +
! align="center" style="background:#f0f0f0;"|<u>1</u>
 +
! align="center" style="background:#f0f0f0;"|<u>2</u>
 +
! align="center" style="background:#f0f0f0;"|<u>3</u>
 +
! align="center" style="background:#f0f0f0;"|<u>4</u>
 +
! align="center" style="background:#f0f0f0;"|<u>5</u>
 +
! align="center" style="background:#f0f0f0;"|<u>6</u>
 +
! align="center" style="background:#f0f0f0;"|<u>7</u>
 +
! align="center" style="background:#f0f0f0;"|<u>8</u>
 +
! align="center" style="background:#f0f0f0;"|<u>9</u>
 +
! align="center" style="background:#f0f0f0;"|<u>10</u>
 +
! align="center" style="background:#f0f0f0;"|<u>11</u>
 +
! align="center" style="background:#f0f0f0;"|<u>12</u>
 +
! align="center" style="background:#f0f0f0;"|<u>13</u>
 +
! align="center" style="background:#f0f0f0;"|<u>14</u>
 +
! align="center" style="background:#f0f0f0;"|<u>15</u>
 +
|-
 +
!Gel<br>Lysates
 +
| align="center"|X
 +
| align="center"|[http://openwetware.org/images/7/74/Spectra_Multicolor_High_Range_Protein_Ladder_-_Fermentas.jpg Fermantas high range Marker]<br> (4 μL)
 +
| align="center"|HeLa -S<br>(40 μL)
 +
| align="center"|Jurkat <br>(40 μL)
 +
| align="center"|HEK293<br>(40 μL)
 +
| align="center"|[http://openwetware.org/images/7/74/Spectra_Multicolor_High_Range_Protein_Ladder_-_Fermentas.jpg Fermantas high range Marker]<br> (10 μL)
 +
| align="center"|S0<br>(10,1 μL)
 +
| align="center"|S1<br>(27,5 μL)
 +
| align="center"|S2<br>(10,8 μL)
 +
| align="center"|S3<br>(17,2 μL)
 +
| align="center"|C0<br>(40,0 μL)
 +
| align="center"|C1<br>(13,8 μL)
 +
| align="center"|C2<br>(38,3 μL)
 +
| align="center"|C3<br>(17,2 μL) 
 +
| align="center"|X
 +
|-
 +
|}
 +
* Gel was cut in 4 pieces and treated with either GAPDH (1:2000, mouse), RII (1:500, rabbit), AKAP79 (1:500, mouse) or Gravin (1:500, rabbit)
<!--
<!--
==Materials & Methods==
==Materials & Methods==

Current revision

Project name Main project page
Previous entry      Next entry

Summary

  • Loading 10% gel
    • Loading volume was corrected according to the quantification of previous blot to obtain equal loading
10% SDS-PAGE Gel loading hTERT D9 + Jurkat, HEK293 & HeLa -S see codes 21June2010 S0 & C0 are of 7.5.2010
# 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
Gel
Lysates
X Fermantas high range Marker
(4 μL)
HeLa -S
(40 μL)
Jurkat
(40 μL)
HEK293
(40 μL)
Fermantas high range Marker
(10 μL)
S0
(10,1 μL)
S1
(27,5 μL)
S2
(10,8 μL)
S3
(17,2 μL)
C0
(40,0 μL)
C1
(13,8 μL)
C2
(38,3 μL)
C3
(17,2 μL)
X
  • Gel was cut in 4 pieces and treated with either GAPDH (1:2000, mouse), RII (1:500, rabbit), AKAP79 (1:500, mouse) or Gravin (1:500, rabbit)


Personal tools