User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/10/18

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< User:Wilfred J. Poppinga | Notebook | cAMP compartmentalization | 2010 | 10(Difference between revisions)
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(Summary)
Current revision (07:35, 19 October 2010) (view source)
m (Summary)
 
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* Loading 10% gel
* Loading 10% gel
** Loading volume was corrected according to the quantification of previous blot to obtain equal loading
** Loading volume was corrected according to the quantification of previous blot to obtain equal loading
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{| {{table}}
 
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| align="center" style="background:#f0f0f0;"|''''''
 
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| align="center" style="background:#f0f0f0;"|'''S0'''
 
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| align="center" style="background:#f0f0f0;"|'''S1'''
 
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| align="center" style="background:#f0f0f0;"|'''S2'''
 
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| align="center" style="background:#f0f0f0;"|'''S3'''
 
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| align="center" style="background:#f0f0f0;"|'''C0'''
 
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| align="center" style="background:#f0f0f0;"|'''C1'''
 
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| align="center" style="background:#f0f0f0;"|'''C2'''
 
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| align="center" style="background:#f0f0f0;"|'''C3'''
 
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|-
 
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| Loading volume (corrected)||||||||||||||||
 
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|}
 
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*
 
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{| border="1"
{| border="1"
|+'''10% SDS-PAGE Gel loading hTERT D9 + Jurkat, HEK293 & HeLa -S see codes [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/21|21June2010]] S0 & C0 are of 7.5.2010'''
|+'''10% SDS-PAGE Gel loading hTERT D9 + Jurkat, HEK293 & HeLa -S see codes [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/21|21June2010]] S0 & C0 are of 7.5.2010'''
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!Gel<br>Lysates
!Gel<br>Lysates
| align="center"|X
| align="center"|X
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| align="center"|[http://openwetware.org/images/7/74/Spectra_Multicolor_High_Range_Protein_Ladder_-_Fermentas.jpg Fermantas high range Marker]<br> (5 μL)
+
| align="center"|[http://openwetware.org/images/7/74/Spectra_Multicolor_High_Range_Protein_Ladder_-_Fermentas.jpg Fermantas high range Marker]<br> (4 μL)
| align="center"|HeLa -S<br>(40 μL)
| align="center"|HeLa -S<br>(40 μL)
| align="center"|Jurkat <br>(40 μL)
| align="center"|Jurkat <br>(40 μL)

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Summary

  • Loading 10% gel
    • Loading volume was corrected according to the quantification of previous blot to obtain equal loading
10% SDS-PAGE Gel loading hTERT D9 + Jurkat, HEK293 & HeLa -S see codes 21June2010 S0 & C0 are of 7.5.2010
# 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
Gel
Lysates
X Fermantas high range Marker
(4 μL)
HeLa -S
(40 μL)
Jurkat
(40 μL)
HEK293
(40 μL)
Fermantas high range Marker
(10 μL)
S0
(10,1 μL)
S1
(27,5 μL)
S2
(10,8 μL)
S3
(17,2 μL)
C0
(40,0 μL)
C1
(13,8 μL)
C2
(38,3 μL)
C3
(17,2 μL)
X
  • Gel was cut in 4 pieces and treated with either GAPDH (1:2000, mouse), RII (1:500, rabbit), AKAP79 (1:500, mouse) or Gravin (1:500, rabbit)


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