User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/10/18

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Loading 10% gel
- Loading volume was corrected according to the quantification of previous blot to obtain equal loading

**10% SDS-PAGE Gel loading hTERT D9 + Jurkat, HEK293 & HeLa -S see codes 21June2010 S0 & C0 are of 7.5.2010**
#	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15
Gel Lysates	X	Fermantas high range Marker (4 μL)	HeLa -S (40 μL)	Jurkat (40 μL)	HEK293 (40 μL)	Fermantas high range Marker (10 μL)	S0 (10,1 μL)	S1 (27,5 μL)	S2 (10,8 μL)	S3 (17,2 μL)	C0 (40,0 μL)	C1 (13,8 μL)	C2 (38,3 μL)	C3 (17,2 μL)	X

Gel was cut in 4 pieces and treated with either GAPDH (1:2000, mouse), RII (1:500, rabbit), AKAP79 (1:500, mouse) or Gravin (1:500, rabbit)

@@ Line 13: / Line 13: @@
 * Loading 10% gel
 ** Loading volume was corrected according to the quantification of previous blot to obtain equal loading
-{| {{table}}
-| align="center" style="background:#f0f0f0;"|''''''
-| align="center" style="background:#f0f0f0;"|'''S0'''
-| align="center" style="background:#f0f0f0;"|'''S1'''
-| align="center" style="background:#f0f0f0;"|'''S2'''
-| align="center" style="background:#f0f0f0;"|'''S3'''
-| align="center" style="background:#f0f0f0;"|'''C0'''
-| align="center" style="background:#f0f0f0;"|'''C1'''
-| align="center" style="background:#f0f0f0;"|'''C2'''
-| align="center" style="background:#f0f0f0;"|'''C3'''
-|-
-| Loading volume (corrected)||||||||||||||||
-|}
-*
 {| border="1"
 |+'''10% SDS-PAGE Gel loading hTERT D9 + Jurkat, HEK293 & HeLa -S see codes [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/21|21June2010]] S0 & C0 are of 7.5.2010'''
@@ Line 49: / Line 34: @@
 !Gel<br>Lysates
 | align="center"|X
-| align="center"|[http://openwetware.org/images/7/74/Spectra_Multicolor_High_Range_Protein_Ladder_-_Fermentas.jpg Fermantas high range Marker]<br> (5 μL)
+| align="center"|[http://openwetware.org/images/7/74/Spectra_Multicolor_High_Range_Protein_Ladder_-_Fermentas.jpg Fermantas high range Marker]<br> (4 μL)
 | align="center"|HeLa -S<br>(40 μL)
 | align="center"|Jurkat <br>(40 μL)