User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/10/19

From OpenWetWare

< User:Wilfred J. Poppinga | Notebook | cAMP compartmentalization | 2010 | 10(Difference between revisions)
Jump to: navigation, search
(Autocreate 2010/10/19 Entry for User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization)
Current revision (03:21, 25 October 2010) (view source)
m (Summary)
 
(2 intermediate revisions not shown.)
Line 11: Line 11:
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Summary==
==Summary==
-
* Short summary
+
* qPCR hTERT, HEK and Jurkat for AKAP9
 +
** 35 reactions (mastermix) (262.5 μL Blue mix, 26.25 μL primer/probe, 201.25 μL H<sub>2</sub>O)
 +
** 14 μL each well + 1 μL cDNA
 +
* Pierce for HBE cells (see attachment)
 +
* Blots of 10% gel
 +
[[Image:19102010_10%25_gel_hTERT.tif|500px]]
<!--
<!--
==Materials & Methods==
==Materials & Methods==
Line 32: Line 37:
===Related topics===
===Related topics===
*
*
 +
-->
 +
==Attachment==
==Attachment==
<html>
<html>
-
<a href="http://openwetware.org/wiki/Image:FILE.xls">
+
<a href="http://openwetware.org/wiki/Image:19102010_Pierce_Lysates_HBE.xls">
   <img src="http://openwetware.org/images/f/f8/Owwnotebook_icon.png" alt="" width="50" height="50">
   <img src="http://openwetware.org/images/f/f8/Owwnotebook_icon.png" alt="" width="50" height="50">
</a>
</a>
-
</html> [[Media:FILE.xls| Excel TITLE]]
+
</html> [[Media:19102010_Pierce_Lysates_HBE.xls| Pierce resultaten HBE (& Jurkat)]]
-
-->
+
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
|}
|}
__NOTOC__
__NOTOC__

Current revision

Project name Main project page
Previous entry      Next entry

Summary

  • qPCR hTERT, HEK and Jurkat for AKAP9
    • 35 reactions (mastermix) (262.5 μL Blue mix, 26.25 μL primer/probe, 201.25 μL H2O)
    • 14 μL each well + 1 μL cDNA
  • Pierce for HBE cells (see attachment)
  • Blots of 10% gel

Attachment

Pierce resultaten HBE (& Jurkat)


Personal tools