User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/10/19

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Current revision (03:21, 25 October 2010) (view source)
m (Summary)
 
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* Pierce for HBE cells (see attachment)
* Pierce for HBE cells (see attachment)
* Blots of 10% gel
* Blots of 10% gel
 +
[[Image:19102010_10%25_gel_hTERT.tif|500px]]
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==Materials & Methods==
==Materials & Methods==

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Summary

  • qPCR hTERT, HEK and Jurkat for AKAP9
    • 35 reactions (mastermix) (262.5 μL Blue mix, 26.25 μL primer/probe, 201.25 μL H2O)
    • 14 μL each well + 1 μL cDNA
  • Pierce for HBE cells (see attachment)
  • Blots of 10% gel

Attachment

Pierce resultaten HBE (& Jurkat)


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