User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/10/19

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==Summary==
==Summary==
* qPCR hTERT, HEK and Jurkat for AKAP9
* qPCR hTERT, HEK and Jurkat for AKAP9
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* Pierce for HBE cells
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** 35 reactions (mastermix) (262.5 μL Blue mix, 26.25 μL primer/probe, 201.25 μL H<sub>2</sub>O)
 +
** 14 μL each well + 1 μL cDNA
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* Pierce for HBE cells (see attachment)
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* Blots of 10% gel
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==Materials & Methods==
==Materials & Methods==
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==Attachment==
==Attachment==
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Revision as of 08:40, 19 October 2010

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Summary

  • qPCR hTERT, HEK and Jurkat for AKAP9
    • 35 reactions (mastermix) (262.5 μL Blue mix, 26.25 μL primer/probe, 201.25 μL H2O)
    • 14 μL each well + 1 μL cDNA
  • Pierce for HBE cells (see attachment)
  • Blots of 10% gel

Attachment

Pierce resultaten HBE (& Jurkat)


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