User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/10/19 - Revision history

Wilfred J. Poppinga: /* Summary */

2010-10-25T08:21:00Z

Summary

←Older revision		Revision as of 08:21, 25 October 2010
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	* Pierce for HBE cells (see attachment)		* Pierce for HBE cells (see attachment)
	* Blots of 10% gel		* Blots of 10% gel
		+	[[Image:19102010_10%25_gel_hTERT.tif\|500px]]
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	==Materials & Methods==		==Materials & Methods==

Wilfred J. Poppinga: /* Summary */

2010-10-19T13:40:34Z

Summary

←Older revision		Revision as of 13:40, 19 October 2010
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	==Summary==		==Summary==
	* qPCR hTERT, HEK and Jurkat for AKAP9		* qPCR hTERT, HEK and Jurkat for AKAP9
-	* Pierce for HBE cells	+	** 35 reactions (mastermix) (262.5 μL Blue mix, 26.25 μL primer/probe, 201.25 μL H<sub>2</sub>O)
		+	** 14 μL each well + 1 μL cDNA
		+	* Pierce for HBE cells (see attachment)
		+	* Blots of 10% gel
	<!--		<!--
	==Materials & Methods==		==Materials & Methods==
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	*		*
	-->		-->
		+
	==Attachment==		==Attachment==
	<html>		<html>

Wilfred J. Poppinga: /* Summary */

2010-10-19T12:43:34Z

Summary

←Older revision		Revision as of 12:43, 19 October 2010
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	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
	==Summary==		==Summary==
-	* ~~Short summary~~	+	* qPCR hTERT, HEK and Jurkat for AKAP9
		+	* Pierce for HBE cells
	<!--		<!--
	==Materials & Methods==		==Materials & Methods==
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	===Related topics===		===Related topics===
	*		*
		+	-->
	==Attachment==		==Attachment==
	<html>		<html>
-	<a href="http://openwetware.org/wiki/Image:~~FILE~~.xls">	+	<a href="http://openwetware.org/wiki/Image:19102010_Pierce_Lysates_HBE.xls">
	<img src="http://openwetware.org/images/f/f8/Owwnotebook_icon.png" alt="" width="50" height="50">		<img src="http://openwetware.org/images/f/f8/Owwnotebook_icon.png" alt="" width="50" height="50">
	</a>		</a>
-	</html> [[Media:~~FILE~~.xls\| ~~Excel TITLE~~]]	+	</html> [[Media:19102010_Pierce_Lysates_HBE.xls\| Pierce resultaten HBE (& Jurkat)]]
-	~~-->~~	+
	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
	\|}		\|}

	__NOTOC__		__NOTOC__

Wilfred J. Poppinga: Autocreate 2010/10/19 Entry for User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization

2010-10-19T12:35:59Z

Autocreate 2010/10/19 Entry for User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Summary==
* Short summary


|}

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