User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/10/20: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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==Summary==
==Summary==
* Short summary
* 6% gel HBE en hTERT (AKAP250(AKAP79))
{| border="1"
|+'''6% SDS-PAGE Gel loading hTERT D9 + Jurkat, HEK293 & HeLa -S see codes [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/21|21June2010]] S0 & C0 are of 7.5.2010'''
! align="center" style="background:#f0f0f0;"|#
! align="center" style="background:#f0f0f0;"|<u>1</u>
! align="center" style="background:#f0f0f0;"|<u>2</u>
! align="center" style="background:#f0f0f0;"|<u>3</u>
! align="center" style="background:#f0f0f0;"|<u>4</u>
! align="center" style="background:#f0f0f0;"|<u>5</u>
! align="center" style="background:#f0f0f0;"|<u>6</u>
! align="center" style="background:#f0f0f0;"|<u>7</u>
! align="center" style="background:#f0f0f0;"|<u>8</u>
! align="center" style="background:#f0f0f0;"|<u>9</u>
! align="center" style="background:#f0f0f0;"|<u>10</u>
! align="center" style="background:#f0f0f0;"|<u>11</u>
! align="center" style="background:#f0f0f0;"|<u>12</u>
! align="center" style="background:#f0f0f0;"|<u>13</u>
! align="center" style="background:#f0f0f0;"|<u>14</u>
! align="center" style="background:#f0f0f0;"|<u>15</u>
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!Gel<br>Lysates
| align="center"|[http://openwetware.org/images/7/74/Spectra_Multicolor_High_Range_Protein_Ladder_-_Fermentas.jpg Fermantas high range Marker]<br> (10 μL)
| align="center"|HeLa -S<br>(40 μL)
| align="center"|Jurkat <br>(40 μL)
| align="center"|HEK293<br>(40 μL)
| align="center"|HBE<br> CTR<br> (20 μL)
| align="center"|HBE<br> 5% CSE (6h)<br> (20 μL)
| align="center"|S0<br>(40 μL)
| align="center"|S1<br>(40 μL)
| align="center"|S2<br>(40 μL)
| align="center"|S3<br>(40 μL)
| align="center"|C0<br>(40 μL)
| align="center"|C1<br>(40 μL)
| align="center"|C2<br>(40 μL)
| align="center"|C3<br>(40 μL) 
| align="center"|X
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* 18S qPCR
* 10% repeat yesterday (new ECL)
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==Materials & Methods==
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===Related topics===
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==Attachment==
==Attachment==
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<html>
<a href="http://openwetware.org/wiki/Image:FILE.xls">
<a href="http://openwetware.org/wiki/Image:19Oct2010_AKAP9_in_hTERT.xls">
   <img src="http://openwetware.org/images/f/f8/Owwnotebook_icon.png" alt="" width="50" height="50">
   <img src="http://openwetware.org/images/f/f8/Owwnotebook_icon.png" alt="" width="50" height="50">
</a>
</a>
</html> [[Media:FILE.xls| Excel TITLE]]
</html> [[Media:19Oct2010_AKAP9_in_hTERT.xls|qPCR results AKAP9 (hTERT)]]
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__NOTOC__
__NOTOC__

Latest revision as of 19:57, 26 September 2017

<html><style type="text/css"> .todo { color: red } .done { color: green} </style></html>

Project name Main project page
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Summary

  • 6% gel HBE en hTERT (AKAP250(AKAP79))
6% SDS-PAGE Gel loading hTERT D9 + Jurkat, HEK293 & HeLa -S see codes 21June2010 S0 & C0 are of 7.5.2010
# 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
Gel
Lysates 		Fermantas high range Marker
(10 μL) 		HeLa -S
(40 μL) 		Jurkat
(40 μL) 		HEK293
(40 μL) 		HBE
CTR
(20 μL) 		HBE
5% CSE (6h)
(20 μL) 		S0
(40 μL) 		S1
(40 μL) 		S2
(40 μL) 		S3
(40 μL) 		C0
(40 μL) 		C1
(40 μL) 		C2
(40 μL) 		C3
(40 μL) 		X
  • 18S qPCR
  • 10% repeat yesterday (new ECL)

Attachment

<html> <a href="http://openwetware.org/wiki/Image:19Oct2010_AKAP9_in_hTERT.xls"> 

  <img src="http://openwetware.org/images/f/f8/Owwnotebook_icon.png" alt="" width="50" height="50">

</a> </html> qPCR results AKAP9 (hTERT)


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